Vitamin B12 and Folate (vitamin B9) and iron are each essential in the manufacture and recycling of red blood cells, and we know that disturbances in iron processing in the body also disturb dopamine production in the brain.
Deficiencies of B12 and Folate have been linked to RLS symptoms and have also been shown to affect the severity of RLS, so that symptoms are worse with lower levels of B12 and Folate.
Low levels of B12/Folate may cause other symptoms such as tiredness, muscle weakness, fatigue and prickling sensations over the skin. These symptoms may be indistinguishable from those of Restless Legs Syndrome itself.
Improvement in B12/Folate status by supplementing with these vitamins has been shown to reduce symptom severity in RLS.